Organic Synthesis of an Unnatural Fluorescent Amino Acid
Dr. Thomas Goyne and Dr. Jeffrey Pruet
Arts and Sciences
The long-term goal of this project is to chemically synthesize an unnatural fluorescent amino acid, 3-[7-nitro-2,1,3-benzoxadiazol-4-yl]-L-alanine. This amino acid can be used to build glow-in-the-dark proteins, allowing investigators to visualize a single protein in an otherwise transparent living cell. We have been unable to replicate the previously reported synthesis and are exploring different reaction conditions and workup procedures. To determine the efficiency of the new reaction conditions, reaction kinetics will be analyzed using 1H-NMR and LCMS, which follow the reactions over time.
Niemeyer, Cassandra, "Organic Synthesis of an Unnatural Fluorescent Amino Acid" (2020). Summer Interdisciplinary Research Symposium. 75.