Organic Synthesis of an Unnatural Fluorescent Amino Acid
Faculty Sponsor
Dr. Thomas Goyne and Dr. Jeffrey Pruet
College
Arts and Sciences
Department/Program
Chemistry
Presentation Type
Poster Presentation
Symposium Date
Summer 7-24-2020
Abstract
The long-term goal of this project is to chemically synthesize an unnatural fluorescent amino acid, 3-[7-nitro-2,1,3-benzoxadiazol-4-yl]-L-alanine. This amino acid can be used to build glow-in-the-dark proteins, allowing investigators to visualize a single protein in an otherwise transparent living cell. We have been unable to replicate the previously reported synthesis and are exploring different reaction conditions and workup procedures. To determine the efficiency of the new reaction conditions, reaction kinetics will be analyzed using 1H-NMR and LCMS, which follow the reactions over time.
Recommended Citation
Niemeyer, Cassandra, "Organic Synthesis of an Unnatural Fluorescent Amino Acid" (2020). Summer Interdisciplinary Research Symposium. 75.
https://scholar.valpo.edu/sires/75
Biographical Information about Author(s)
Cassandra Niemeyer is a senior at Valparaiso University. She is also a Biochemistry major pursuing premed.