Development of Improved Thiol-Based Fluorescent Probes for the Detection of HNO in Biological Systems

Primary Submission Contact

Dr. Jefferson Chan

Faculty Sponsor

Dr. Jefferson Chan

Faculty Sponsor Email Address

[email protected]

College

Arts and Sciences

Department/Program

Chemistry

Document Type

Poster Presentation

Date

Fall 10-26-2018

Abstract

Nitroxyl (HNO) has gained attention recently for its role as a therapeutic agent for cardiovascular disorders and as a potential anticancer agent. NitroxylFluor is a recently developed thiol-based molecular probe that reacts with HNO to release a fluorescein derivative. The goals of this project were to develop a diethyl version of NitroxylFluor that reacts with HNO more rapidly, minimizing unwanted side reactions, as well as a generalizable trigger that could be used with a wide variety of fluorescent dye platforms. We were able to confirm that a coumarin-based probe is too labile to be used in vivo without additional synthetic modifications. Additionally, we made progress in developing a diethyl fluorescein-based probe that could potentially be coupled with the generalizable trigger to allow for a wide range of dyes to be used in our probes for the detection of HNO.

Biographical Information about Author(s)

I (Mara Paterson) worked on this project at the University of Illinois at Urbana-Champaign during the summer of 2018 as a part of the Snyder Scholar program. I am interested in organic chemistry and chemical biology, and I plan to attend graduate school to study one of those two fields. Ultimately, I hope to become an organic chemistry professor at a small, primarily undergraduate institution.

Additional Presentation Information

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