Title

LAP3, a novel plant protein required for pollen development, is essential for proper exine formation

Authors

Anna A. Dobritsa, University of Chicago
Shuh-Ichi Nishikawa, Nagoya University
Daphne Preuss, University of Chicago
Ewa Urbanczyk-Wochniak, Samuel Roberts Noble Foundation
Lloyd W. Sumner, Samuel Roberts Noble Foundation
Adam Hammond, University of Chicago
Ann L. Carlson, Valparaiso University
Rob Swanson, Valparaiso UniversityFollow

Document Type

Article

Publication Date

2009

Abstract

We isolated lap3-1 and lap3-2 mutants in ascreen for pollen that displays abnormal stigma binding.Unlike wild-type pollen, lap3-1 and lap3-2 pollen exine isthinner, weaker, and is missing some connections betweentheir roof-like tectum structures. We describe the mappingand identification of LAP3 as a novel gene that contains arepetitive motif found in b-propeller enzymes. Insertionmutations in LAP3 lead to male sterility. To investigatepossible roles for LAP3 in pollen development, we assayedthe metabolite profile of anther tissues containing developingpollen grains and found that the lap3-2 defect leadsto a broad range of metabolic changes. The largest changeswere seen in levels of a straight-chain hydrocarbon nonacosaneand in naringenin chalcone, an obligate compoundin the flavonoid biosynthesis pathway.

Recommended Citation

Dobritsa, A. A., Nishikawa, S. I., Preuss, D., Urbanczyk-Wochniak, E., Sumner, L. W., Hammond, A., Carlson, A. L. & Swanson, R. J. 2009 LAP3, a novel plant protein required for pollen development, is essential for proper exine formation. Sexual Plant Reproduction 22: 167-177.