Leaves of Populus tremuloides and Salix babylonica held in the laboratory as for feeding insects were analyzed for total nitrogen, total phenolics, and total sugars at 0, 3, and 6 days. More often than not, the three components varied due to leaf age, time since excision, or temperature. Temporal changes altered nutritional balance and probable impact on insect performance, Results suggest that other woody plant species would not remain nutritionally stable for long in the laboratory,
Miller, William E.
"Change in Nutritional Quality of Detached Aspen and Willow Foliage Used as Insect Food in the Laboratory,"
The Great Lakes Entomologist, vol 20
Available at: https://scholar.valpo.edu/tgle/vol20/iss1/4