The Synthesis of Unnatural Amino Acids
Dr. Thomas Goyne
Arts and Sciences
The long-term goal of this project is to chemically synthesize an unnatural fluorescent amino acid (UFAA) that can later be used to build glow-in-the-dark proteins. UFAAs allow investigators to visualize a single protein in an otherwise transparent living cell. The specific UFAA target for this project is L-alanine, 3-[7-nitro-2,1,3-benzoxadiazol-4-yl], which is an analog of the natural amino acid tryptophan. This synthesis consists of a coupling reaction followed by a deprotection reaction. Products have been characterized using 1H and 13C NMR, and Liquid Chromatography Spectrometry (LC-MS). Future tasks could include optimizing the yield and purity of this UFAA.
Johnson, Madeline, "The Synthesis of Unnatural Amino Acids" (2018). Summer Interdisciplinary Research Symposium. 22.