The Genetic Analysis of Cortical Bone Morphology in Mice
Dr. Jane Kenney-Hunt
Arts and Sciences
Department of Biology
Osteoporosis is a disease that affects bone mass and thickness, which ultimately leads to bone fracture. It is known that decreased cortical thickness and bone area are associated with the development of osteoporosis. We analyzed genetic effects on cortical bone thickness, cortical bone area and polar moment of inertia in the femora and radii of 17 recombinant inbred mouse lines. The quantitative trait locus (QTL) analysis of the recombinant mice indicates regions of the genome that have effects on the specific trait. Identification of QTL in sex and diet isolated experiments can indicate gene by environment interactions. 423 mice from 17 LG/J and SM/J cross lines were provided with either a low-fat or high-fat diet for 20 weeks. After necropsy, bone area and thickness were collected from microCT images. Results from genetic analysis revealed three QTL affecting bone area, three QTL determining cortical thickness and four QTL that influence polar moment of inertia. These QTL demonstrate the genetic effects on bone structure interacting with the effects of dietary fat and sex.
Martens, Sydney and Kenney-Hunt, Jane, "The Genetic Analysis of Cortical Bone Morphology in Mice" (2021). Summer Interdisciplinary Research Symposium. 112.