Analysis of the Popliteal Lymph Node as a Biomarker to Monitor Arthritic Flare in Male versus Female Tumor Necrosis Factor-Transgenic Mice with Inflammatory Arthritis
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Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by inflammation in peripheral synovial joint tissue and is suggested to be more severe in women. The popliteal lymph node (PLN) has been found to be a biomarker of arthritic flare in the human TNF-α transgenic (TNF-Tg) mouse model of inflammatory arthritis. The goal of this study was to verify that anti-TNF therapy is equally effective in reducing the severity of arthritis in male and female TNF-Tg mice using PLN volume as a marker of arthritic flare.
Male and female TNF-Tg mice were subdivided into two groups and treated with either placebo or anti-TNF therapy for six weeks. Power-Doppler (PD) ultrasound imaging was taken of right and left PLNs in each subgroup (n=6 per subgroup). 3D reconstructions were created to determine PLN total volume, PD volume, and normalized PD volume. Knee tissue was collected at the end of treatment for histologic analysis (n=6 per subgroup).
There was a decrease in the PLN volumes in the anti-TNF treated groups compared to the placebo-treated groups for both sexes (p
Overall, increased PLN size positively correlates to joint inflammation and serves as an appropriate biomarker for arthritic flare. Furthermore, anti-TNF therapy was equally effective in decreasing arthritic severity in males and female TNF-Tg mice compared to placebo-treated mice of both sexes.
Henkes, Zoe I., "Analysis of the Popliteal Lymph Node as a Biomarker to Monitor Arthritic Flare in Male versus Female Tumor Necrosis Factor-Transgenic Mice with Inflammatory Arthritis" (2018). Fall Interdisciplinary Research Symposium. 99.
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