Development of Improved Thiol-Based Fluorescent Probes for the Detection of HNO in Biological Systems

Primary Submission Contact

Dr. Jefferson Chan

Faculty Sponsor

Dr. Jefferson Chan

Faculty Sponsor Email Address



Arts and Sciences



Document Type

Poster Presentation


Fall 10-26-2018


Nitroxyl (HNO) has gained attention recently for its role as a therapeutic agent for cardiovascular disorders and as a potential anticancer agent. NitroxylFluor is a recently developed thiol-based molecular probe that reacts with HNO to release a fluorescein derivative. The goals of this project were to develop a diethyl version of NitroxylFluor that reacts with HNO more rapidly, minimizing unwanted side reactions, as well as a generalizable trigger that could be used with a wide variety of fluorescent dye platforms. We were able to confirm that a coumarin-based probe is too labile to be used in vivo without additional synthetic modifications. Additionally, we made progress in developing a diethyl fluorescein-based probe that could potentially be coupled with the generalizable trigger to allow for a wide range of dyes to be used in our probes for the detection of HNO.

Biographical Information about Author(s)

I (Mara Paterson) worked on this project at the University of Illinois at Urbana-Champaign during the summer of 2018 as a part of the Snyder Scholar program. I am interested in organic chemistry and chemical biology, and I plan to attend graduate school to study one of those two fields. Ultimately, I hope to become an organic chemistry professor at a small, primarily undergraduate institution.

Additional Presentation Information

Wall Poster

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