Does PKCβII Modulate Colon Cell Growth?

Primary Submission Contact

Lauren Hargrave

Faculty Sponsor

Beth Scaglione-Sewell

Faculty Sponsor Email Address



Arts and Sciences



Document Type

Poster Presentation


Fall 9-12-2014


It is known that colon carcinoma tissues contain higher levels of Protein Kinase C beta II (PKCβII) in comparison to surrounding non-involved tissue. A study is being conducted to determine if PKCβII is a causative step in or effect of colon tumor formation. The PKCβII deoxyribose-nucleic acid (DNA) sequence has been sub-cloned into the pENTR™ vector. The PKCβII is then sub-cloned into pT-Rex™-DEST31 vector, which carries a inducible promoter. Site-directed mutagenesis has been carried out on the PKCβII in order to determine the role of the kinase activity in tumor formation. KpnI restriction enzyme has been used to verify that PKCβII is present in the correct locations of the pT-Rex™-DEST31plasmid. Studies continue to be conducted to search for the role of PKCβII in colon tumor formation.

Biographical Information about Author(s)

Lauren Hargrave is a Biology major, Chemistry minor, and Christ College student. She hopes to attend graduate school to become a Physician Assistant.

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