Does PKCβII Modulate Colon Cell Growth?
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It is known that colon carcinoma tissues contain higher levels of Protein Kinase C beta II (PKCβII) in comparison to surrounding non-involved tissue. A study is being conducted to determine if PKCβII is a causative step in or effect of colon tumor formation. The PKCβII deoxyribose-nucleic acid (DNA) sequence has been sub-cloned into the pENTR™ vector. The PKCβII is then sub-cloned into pT-Rex™-DEST31 vector, which carries a inducible promoter. Site-directed mutagenesis has been carried out on the PKCβII in order to determine the role of the kinase activity in tumor formation. KpnI restriction enzyme has been used to verify that PKCβII is present in the correct locations of the pT-Rex™-DEST31plasmid. Studies continue to be conducted to search for the role of PKCβII in colon tumor formation.
Hargrave, Lauren, "Does PKCβII Modulate Colon Cell Growth?" (2014). Fall Interdisciplinary Research Symposium. 73.
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