In preliminary screens, several functions, most notably, genes involved in the control of reactive oxygen species (ROS), were identified as playing a role in the process of growth rate compensation of branch density. The maintenance of branch density under growth at various temperatures was examined in a selection of mutants in genes known to be important in the control of ROS. In all ROS control mutants tested, growth was shown to branch tighter when grown at higher temperatures (which result in faster growth rates) and looser when grown at lower temperatures (which results in slower growth rates). This can be contrasted with wild-type Neurospora which branches at the same density under both conditions. We also tested the impact of environmental agents which lower the concentration of ROS on branching. In tests, various water soluble anti-oxidants (ie reducing agents); Ascorbic Acid (aka Vitamin C) and Glutathione produced unusual bra nching patterns in wild-type Neurospora strains exposed.
Yablonowski, Jacob; Zapata, Alex; and Watters, Michael, "Evidence for the impact of Reactive oxygen species on the control of branching in Neurospora crassa" (2011). Symposium on Undergraduate Research and Creative Expression (SOURCE). 89.