Validating Methods to Further Research of the Circadian Rhythm of Saccharomyces cerevisiae
Dr. Sara Dick
Arts and Sciences
Saccharomyces cerevisiae is a common model organism, however there is not a large amount of information covering the circadian rhythms of the organism. In previous work, samples have been collected and killed using several chemical combinations. In order to obtain information concerning the circadian rhythm, these samples need to be tested for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and other enzymatic activity using carious forms of assays that display the amount of activity exhibited from the presence of GAPDH. A method was constructed using a series of tests on samples containing no additional chemicals. The first test that was performed was a Coomassie Bradford Protocol. This quantified the amount of proteins present within the samples. From there, a Western Blot was performed as well as a number of enzyme lined immunosorbent assays (ELISAs). Once a method is determined that preserves the proteins from the cells and is time and cost efficient, the samples collected containing the chemicals can be tested.
Smith, Ashley L., "Validating Methods to Further Research of the Circadian Rhythm of Saccharomyces cerevisiae" (2016). Symposium on Undergraduate Research and Creative Expression (SOURCE). 565.