The Influence of pH Variation on CooA Activity
Dr. Robert Clark
Arts and Sciences
0000-0003-3030-725X (Rachael DeVries), 0000-0001-6991-014X (Brian Weaver)
A bacterial heme protein, CooA, changes shape to bind DNA and activate transcription. Previous research indicated gas molecule (e.g. carbon monoxide) binding to CooA is essential to initiate protein shape change, but recent studies suggested the protein may be able to bind DNA without gas in acidic conditions. To investigate this, CooA was subjected to an acid range of 4-10 pH units and DNA binding was measured via a fluorescence anisotropy assay. From these studies, pH dependent DNA binding was observed, and optimal binding was achieved in the pH 4-6 range. Addition of gas did not improve DNA binding at these pH values. Ongoing studies are investigating the molecular basis of this effect.
DeVries, Rachael M. and Weaver, Brian R., "The Influence of pH Variation on CooA Activity" (2015). Symposium on Undergraduate Research and Creative Expression (SOURCE). 481.