Characterizing the three GAPDH in Saccharomyces cerevisiae
Arts and Sciences
Circadian rhythms are internal signals that follow a 24-hour cycle. They are said to be essential because they cause patterns of humans, animals, plants, and many other organisms. However, the circadian rhythms of all organisms work very differently from one another. Although much is known about the organism Saccharomyces cerevisiae, yeast, not much is know about its circadian rhythm. Numerous studies suggest that Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is an output of the clock in many organisms. Thus, yeast probably produces GAPDH as a signal of what the clock is doing. Yeast has three different genes encoding GAPDH called TDH 1, TDH 2, and TDH 3, which were compared to a known amount of a purchased GAPDH to determine their concentration. Then we used a Western blot to determine that the antibody recognized TDH 1 and TDH 2. These samples then underwent an ELISA to determine the concentration of GAPDH in micrograms/mL. These TDH standards will allow us to show that GAPDH is an output of the clock in yeast and also to study the clock by its effects on this output. Documenting what GAPDH does over time allows for the clock of yeast to be studied in future work.
Fernandez, Jackeline and Tres, Samantha A., "Characterizing the three GAPDH in Saccharomyces cerevisiae" (2014). Symposium on Undergraduate Research and Creative Expression (SOURCE). 380.