Design of Fluorescence Marker-Enzyme Fusion Proteins
Level of Education of Students Involved
Arts and Sciences
In this project, the bacterium Escherichia coli will be used to produce fusion proteins consisting of a fluorescence marker linked to an enzyme. One fusion protein will combine superfolder green fluorescence protein (sfGFP) with glutathione s-transferase (GST), and the other will combine sfGFP with folylpolyglutamate synthase (FPGS). The first stage of this process involves designing a DNA sequence with appropriate linkers, tags, and cleavage sites to be inserted into a plasmid. Then the fusion proteins will be expressed in E. coli, isolated, and finally validated by SDS-PAGE. In the future, other fusion proteins using variants of GST and FPGS will be created.
Nietzel, Maya and Garcia, Amber M., "Design of Fluorescence Marker-Enzyme Fusion Proteins" (2023). Symposium on Undergraduate Research and Creative Expression (SOURCE). 1166.