Level of Education of Students Involved
Michael K. Watters
Arts and Sciences
The control of branching in Neurospora crassa has been studied under numerous environmental conditions. Here we present the observations of N. crassa morphology in simulated low-gravity across two main experiments. Wildtype samples were grown on solid and liquid media. The control group was grown on the lab bench while the experimental group was grown in a clinostat. In the first experiment, the samples were allowed to grow for two days so that the edges of growth could be observed. Qualitative properties including density and growth patterns were first compared. Photographs of microscope images were then used to measure branch intervals, frequency of branching, and branch types. In the second experiment, growth rates were calculated using the amount of growth every 24 hours over four days. Using statistical analysis, it was found that lateral branching, branch intervals, and growth rates were not significantly different between control and experimental samples. However, the growth patterns and density variations were dissimilar, and significant difference was found with the number of main hyphae and apical branching. It was concluded that simulated low-gravity has some effects on the morphology of N. crassa.
Nietzel, Maya and Steverson, Leanna, "Morphological Effects of Simulated Low-Gravity in Neurospora crassa" (2023). Symposium on Undergraduate Research and Creative Expression (SOURCE). 1156.