Title

Synthesis of Vitamin B-9 Derivative

Faculty Sponsor

Thomas Goyne

College

Arts and Sciences

Discipline(s)

Biochemistry

Presentation Type

Poster Presentation

Symposium Date

Spring 4-28-2022

Abstract

The goal of this research is to develop an inexpensive synthesis of the polyglutamated derivative of 5-methyltetrahydrofolate (5MeTHF), commonly known as vitamin B-9. Specifically, the enzyme folyl polyglutamate synthase (FPGS) will be used to catalyze the addition of multiple glutamate residues to the commercially available 5-methyltetrahydrofolate (5MeTHF). A His-tagged variant of the FPGS enzyme from the thermophile b. Licheniformis that was previously added to a plasmid and was expressed in E. coli. The enzyme was purified using a nickel chelate column and then characterized using electrophoresis and liquid chromatography - mass spectroscopy (LC-MS). The LC-MS results confirmed that the addition of glutamate residues to 5MeTHF was successful.

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