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Document Type

Entomological Note

Abstract

(excerpt)

In recent years there has been a burgeoning interest in insect cytogenetics, sometimes involving in vivo cultures of haematocytes for chromosomal analysis. Mitotic poisons, such as colchicine (Tyrkus, 1971), are commonly injected to produce metaphase plates. Likewise, injection of toxins is now common-place in applied insect research. However, surprisingly little general information on injection is available in the literature. The dictates of morphology determine the gross procedure to be used. The kind of needle and syringe, the amount of fluid to be administered, and the necessity of optical aids are a function of the size of the insect recipient. Once these decisions are made, other considerations must still be weighed, including comparative exoskeletal toughness and the insect's stage of development, which are important in determining possible areas for needle penetration.

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