Does PKC-beta II Modulate Colon Cell Growth?
Faculty Sponsor
Beth Scaglione-Sewell
College
Arts and Sciences
Discipline(s)
Biology
Presentation Type
Poster Presentation
Symposium Date
Summer 7-31-2014
Abstract
It has been shown that Protein Kinase C Beta II (PKCβII) is over expressed and more active in colon carcinoma tissues in comparison to surrounding non-involved tissues. A study is being conducted to determine if PKCβII is a causative step in or effect of colon tumor formation. The PKCβII deoxyribose-nucleic acid (DNA) sequence has been sub-cloned into an Entry vector with a inducible promoter. Site-directed mutagenesis had been carried out on the PKCβII kinase domain in order to allow for studies to be conducted on the role of PKCβII kinase activity in colon tumors. The PKCβII has been sub-cloned from the Entry vector into a pT-Rex-DEST31 vector also with a inducible promoter. KPNI restriction enzyme has been used to verify that PKCβII is present in the correct locations of the pT-Rex-DEST31 plasmid.
Recommended Citation
Hargrave, Lauren and Sopetti, Michelle, "Does PKC-beta II Modulate Colon Cell Growth?" (2014). Symposium on Undergraduate Research and Creative Expression (SOURCE). 392.
https://scholar.valpo.edu/cus/392
Biographical Information about Author(s)
Lauren Hargrave is a biology major, chemistry minor, and Christ College associate. She hopes to attend graduate school to become a physician assistant.
Michelle Sopetti is a biology major, psychology minor, Christ College associate. She plans to attend graduate school to become a doctor of physical therapy.