Faculty Sponsor

Rob Swanson

College

Arts and Sciences

Department/Program

Biology

Document Type

Poster Presentation

Celebration Date

5-3-2014

Abstract

Seed number per fruit is a vitally important trait because it determines total agricultural yield. Despite its importance, the genes that direct seed yield remain relatively unknown. We are using Recombinant Inbred Lines (RILs) in the weedy plant Arabidopsis thaliana to explore the heritability of seed yield and genetically map the genes responsible. Up to eight fruits were collected per plant from each RIL. Seeds were scanned using a flatbed scanner and counted using Adobe Photoshop CS6. With this data, we performed an ANOVA to calculate heritability, the amount of variance in the seed yield trait due to genetics. The heritability of seed yield was found to be 23.24 percent. Using the same data set, we are currently performing composite interval mapping to identify the genomic regions that contain the genes that direct seed yield, an analysis we will report on. Locating the genomic regions that contain seed yield is the first vital step in defining the gene network that directs seed number in fruit.

Biographical Information about Author(s)

David Goad is a senior biology major who has been working with Dr. Swanson for three years. For two of those years, the team worked to identify the genes responsible for non-random mating in Arabidopsis. Last fall, David switched to the current project to try his hand at genetic mapping. Next year, David will be attending Washington University in St. Louis to pursue a Ph.D. in Evolution, Ecology, and Population Biology.

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